Creative Biogene CRISPR/Cas9 Platform provides a HIEF™ site-specific gene knock-in service for customers. Based on our CRISPR/Cas9 platform, we developed a HIRF™ site-specific knock-in technology, with which single-copy target gene can be integrated into safe harbor locus (like Rosa26 locus). This technology shows a very high efficiency with or without clonal selection when integrating a single copy. You can expect that a high-yield cell line for bioproduction be received from Creative Biogene.
Integrating exogenous gene in cells (like CHO cells) is widely applied for research and industry, such as recombinant protein production and antibody production. There are three popular methods for gene integration, random insertion, knock-in by nucleases and site specific knock-in. Random insertion is uncertain in gene copies, insertion site and protein yield, and always is not reproductive. A single copy gene can be inserted into specific loci by nucleases, but always with low efficiency and long time. Comparing with the other methods, our HIEF™ site-specific knock-in method is easier and more efficient. HIEF™ site-specific knock-in method is based on recombination, so it is precise insertion and without off-target effects. When Donor plasmids is transfected into cells, recombination will occur under the control of specific enzyme.
CRISPR/Cas9 PlatformCB provides one-stop gene knockin cell line generation service, including reporter gene knockin, tag knockin and fragment knockin. This service ranges from design of gRNA, vector construction, cell transfection and selection, to single clone sequencing and cell expansion. Our talented scientists are expert in knockin cell line generation by using CRISPR system. At CRISPR/Cas9 platformCB, you can expect to achieve with satisfying services and products.
Knockin cell line, which inserted a certain fragment in a...
New Report to Share The Specific Application of GFP Reporter Cell Lines Offered by Creative Biogene
Creative Biogene, as a professional biotechnology organization, having established a large number of reporter stable cell lines suitable for multiple applications. In the studies of life science and biotechnology, the commonly used reporter genes are visually identifiable including green fluorescent protein (GFP), red fluorescent protein (RFP), luciferase, secreted alkaline phosphatase (SEAP) etc. This new report will focus on sharing the application of GFP offered by...
CRISPR/Cas9 PlatformCB utilizes genome editing technology to provide gene knockout and base editing services for customers. Various species are available, including but not limited to Arabidopsis, tobacco, B. distachyum and medicago. As a leading-edge platform around the world, we have experienced scientists, skilled staff and remarkable technology system. CRISPR/Cas9 PlatformCB will assist you with reliable and professional services of plant genome editing.
Model plant is a species that has been widely studied. With its characteristics like dwarf phenotype, short life cycle, fertility and small genome, model plant plays a crucial role in plant genomics research. Model plant is highly homologous with other complex plants in molecular level. Therefore, genome research of model plant establishes a foundation to reveal the functional genomics of higher plant.
CRISPR/Cas9 PlatformCB provides a point mutation cell line generation service for various cell lines, such as tumor cell lines and stem cell lines. As a provider of genome editing service, CRISPR/Cas9 PlatformCB focuses on using the latest technique to serve customers. With years of experience, our scientist team is expert in generating knock-in stable cell lines with CRISPR. At CRISPR/Cas9 PlatformCB, all projects are done by well-trained staff with excellent platform under the guide of talented...
