CRISPR/Cas9 PlatformCB utilizes genome editing technology to provide gene knockout and base editing services for customers. Various species are available, including but not limited to Arabidopsis, tobacco, B. distachyum and medicago. As a leading-edge platform around the world, we have experienced scientists, skilled staff and remarkable technology system. CRISPR/Cas9 PlatformCB will assist you with reliable and professional services of plant genome editing.
Model plant is a species that has been widely studied. With its characteristics like dwarf phenotype, short life cycle, fertility and small genome, model plant plays a crucial role in plant genomics research. Model plant is highly homologous with other complex plants in molecular level. Therefore, genome research of model plant establishes a foundation to reveal the functional genomics of higher plant.
Creative Biogene CRISPR/Cas9 Platform provides a HIEF™ site-specific gene knock-in service for customers. Based on our CRISPR/Cas9 platform, we developed a HIRF™ site-specific knock-in technology, with which single-copy target gene can be integrated into safe harbor locus (like Rosa26 locus). This technology shows a very high efficiency with or without clonal selection when integrating a single copy. You can expect that a high-yield cell line for bioproduction be received from Creative Biogene.
Integrating exogenous gene in cells (like CHO cells) is widely applied for research and industry, such as recombinant protein production and antibody production. There are three popular methods for gene integration, random insertion, knock-in by nucleases and site specific knock-in. Random insertion is uncertain in gene copies, insertion site and protein yield, and always is not reproductive. A single copy gene can be inserted into specific loci by nucleases, but always with low efficiency and long time. Comparing with the other methods, our HIEF™ site-specific knock-in method is easier and more efficient. HIEF™ site-specific knock-in method is based on recombination, so it is precise insertion and without off-target effects. When Donor plasmids is transfected into cells, recombination will occur under the control of specific enzyme.
CRISPR/Cas9 PlatformCB provides a point mutation cell line generation service for various cell lines, such as tumor cell lines and stem cell lines. As a provider of genome editing service, CRISPR/Cas9 PlatformCB focuses on using the latest technique to serve customers. With years of experience, our scientist team is expert in generating knock-in stable cell lines with CRISPR. At CRISPR/Cas9 PlatformCB, all projects are done by well-trained staff with excellent platform under the guide of talented scientists. We provide customers with reliable results and products.
Gene mutation is an important inducer for many diseases. With CRISPR/Cas9 system, it becomes possible to correct the mutated site in living cells. This technique is based on two factors, guide RNA and Cas9 nuclease. Guide RNA alters its sequence for different target sites, while Cas9 functions as a nuclease cutting double strand DNA. At the presence of template, homology directed repair pathway is activated for reconstructing the target site. Due to its versatility, CRISPR system is widely used all over the world and promotes the development of many research fields.
CRISPR/Cas9 PlatformCB provides one-stop gene knockin cell line generation service, including reporter gene knockin, tag knockin and fragment knockin. This service ranges from design of gRNA, vector construction, cell transfection and selection, to single clone sequencing and cell expansion. Our talented scientists are expert in knockin cell line generation by using CRISPR system. At CRISPR/Cas9 platformCB, you can expect to achieve with satisfying services and products.
Knockin cell line, which inserted a certain fragment in a specific locus, is a useful tool for research. When a reporter gene or tag is inserted at the specific locus, this cell line can be used to study endogenous expression level of targeted gene. With the help of CRISPR/Cas9 system, it become easier to generate a custom knockin cell line. Cas9 nuclease targets to different loci of genome through sgRNA with different sequences and then the enzyme introduces double strand break. At the presence of template, DNA double strand break can be repaired via HDR pathway. By this method, promoter, tag or gene can be inserted into genome.
New Report to Share The Specific Application of GFP Reporter Cell Lines Offered by Creative Biogene
Creative Biogene, as a professional biotechnology organization, having established a large number of reporter stable cell lines suitable for multiple applications. In the studies of life science and biotechnology, the commonly used reporter genes are visually identifiable including green fluorescent protein (GFP), red fluorescent protein (RFP), luciferase, secreted alkaline phosphatase (SEAP) etc. This new report will focus on sharing the application of GFP offered by Creative Biogene.
GFP has been considered as a marker for gene expression and protein targeting in intact cells. In biological research, it is widely used as a genetically encoded fluorescent marker. This fluorescent label enables multicolor labeling and is used to study protein-protein interactions. GFP and its derivatives with different colors can be used for a variety of applications, such as studying the function and organization of living systems in protein tags, examining gene expression and various biological options. GFP provides researchers with a rich palette of different spectral and biochemical characteristics of derivatives.
The Application in Cell biology and biotechnology
Since the advent of GFP (autofluorescent protein), whole living cells or organisms have been used as experimental systems in a wide range of fields (cell biology and biomedical). Since 1994, GFP has served as a scientist's agent in the field of biotechnology because it only requires oxygen and energy to function. Fluorescent proteins have become the first line of investigation in the infinite area. It is used in various applications of biotechnology, such as protein fusion, imaging whole organisms and transcription reporters.